Recombinant Human Anti-Streptococcus pneumoniae 23F Antibody (023.102)
CAT#: PABX-173
Anti-Streptococcus pneumoniae 23F antibody is a Human antibody of IgG class that binds to an Streptococcus pneumoniae 23F.
Published Data
Tested Data
Figure 1 Structures of Abs 023.102, pn132p2C05, and KE5 with bound ligands.
The methylated core disaccharide RGP bound to the paratope of Ab 023.102.
Bryson, S., Thomson, C. A., Risnes, L. F., Dasgupta, S., Smith, K., Schrader, J. W., & Pai, E. F. (2016). Structures of Preferred Human IgV Genes–Based Protective Antibodies Identify How Conserved Residues Contact Diverse Antigens and Assign Source of Specificity to CDR3 Loop Variation. The Journal of Immunology, 196(11), 4723-4730.
Figure 2 Germline V-gene–encoded Igs derived from the hypermutated anti-23F Ab (023.102) bind specifically to the 23F polysaccharide in ELISA; importance of the CDRH3 and CDRL3.
A, Left panel, Binding at high concentrations of S.sJ1, which uses the germline sequences of IGHV3-30, IGHJ4, IGKV3-11, and IGKJ1, but with the CDRH3 seen in the hypermutated anti-23F Ab (023.102). Note that binding is observed at far lower concentrations by S.sJ1Ala with a single mutation of Trp to Ala in 96(L) of CDRL3. Right panel, Binding at high concentrations of S.mJ5 using the L chain of the germline Vgene-encoded ancestor of an anti–AD-2S1 Ab and the germline IGKJ5 with a small hydrophilic residue at 96(L) but lacking the additional junctional residue Asp95b(L), whereas, S.sJ5Gly, which has the additional Asp95b(L) binds at lower concentrations to the 23F PPS. No binding of M2.sJ1Ala is observed that has the M2 H chain with the CDRH3 of the germline V-gene–encoded anti–AD-2S1 Abs. Data are the mean 6 SD of triplicate measurements. B, Failure of the germline V-gene–encoded Abs S.sJ1, S.sJ1Ala, S.sJ5Gly, S.mJ5, or M2.sJ1Ala to bind to the 14 PPS (left panel) or 6B PPS (right panel). C, Failure of the germline V-gene–encoded Abs S.sJ1, S.sJ1Ala, S.sJ5Gly, S.mJ5, and M2.sJ1Ala to bind the AD-2S1 peptide. KE5, the hypermutated anti-AD-2S1 mAb, is shown as a positive control. D, A mouse IgM mAb against the human IgG CH3 domain, which forms complexes with S.sJ1 and S.sJ1Ala, increases their avidity for 23F in ELISA. There was no binding observed by the anti–AD-2S1 Ab KE5, either alone or complexed with the IgM. Binding also was not observed with IgM alone (data not shown).
Thomson, C. A., Little, K. Q., Reason, D. C., & Schrader, J. W. (2011). Somatic diversity in CDR3 loops allows single V-genes to encode innate immunological memories for multiple pathogens. The Journal of Immunology, 186(4), 2291-2298.
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SDS-PAGE
Figure 1 Recombinant Mouse Anti-Streptococcus pneumoniae 23F Antibody (PABX-173) in SDS-PAGE
SDS-PAGE analysis of PABX-173 in non-reduced (Lane 2) and reduced (Lane 1) conditions.
SEC-HPLC
Figure 2 Recombinant Mouse Anti-Streptococcus pneumoniae 23F Antibody (PABX-173) in HPLC
HPLC analysis of PABX-173 shows the purity is>95%.
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Specifications
- Immunogen
- The details of the immunogen for this antibody are not available.
- Host Species
- Human
- Derivation
- Human
- Type
- IgG
- Specificity
- Tested positive against native Streptococcus pneumoniae 23F
- Species Reactivity
- Streptococcus pneumoniae
- Clone
- 023. 102
- Applications
- ELISA 1:10000-1:20000
Neut (assay dependent)
FuncS (assay dependent)
Product Property
- Purity
- >95% by SDS-PAGE and HPLC analysis
- Storage
- Store the antibody (in aliquots) at -20°C. Avoid repeated freezing and thawing of samples.
Applications
- Application Notes
- Assay-depended
Target
- Alternative Names
- 23F strep; Streptococcus pneumoniae type 23F
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There are currently no Customer reviews or questions for PABX-173. Click the button above to contact us or submit your feedback about this product.
Very Helpful Product
I recently ordered this antibody to study Streptococcus pneumoniae in my research projects. The high purity and specific binding properties have helped me recognize the 23F antigen easily. I use it in several assays like functional studies and ELISA, and it has not disappointed me. The quality control details in the datasheet are reassuring. This product helps me save time by providing accurate data, which is essential for my research studies.
Reliable and Consistent
I have found this antibody to be very reliable in my research on bacterial antigens. It is particularly effective when detecting the 23F antigen on Streptococcus pneumoniae. I value its consistent performance across different batches and the high purity rate that ensures minimal background noise in experiments. Whether used for ELISA or neutralization test, this product consistently gives clear and reproducible results, making it a staple in my laboratory work.
Solid Performance Overall
I have been very pleased with the performance of the Anti-Streptococcus pneumoniae 23F Antibody. It is easy to work with and provides reliable data when detecting the specific antigen in bacterial studies. The antibody's high specificity reduces error and minimizes background signal, which is essential for clear imaging and analysis in my research. I have recommended this to colleagues, and it has helped streamline our project timelines with its steady performance.
Q&As
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How does this antibody help in research on bacterial targets?
A: This antibody binds tightly to the unique 23F polysaccharide on Streptococcus pneumoniae. Researchers like me find this property very valuable in experiments that focus on bacterial identification and vaccine studies. The antibody provides specific binding, which reduces background noise in assays. I have used it in studies to compare bacterial strains and detect differences in expression. The clear signal it produces is very helpful for accurate data analysis, making it an essential tool in our research.
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What can you tell me about the purity and storage of this antibody?
A: The antibody comes with a purity level over 95%, which is confirmed using SDS-PAGE techniques. This high purity is important for my work since it minimizes interference from unwanted proteins. The product instructions recommend storing it at -20°C, and it remains stable for at least one year. If you reconstitute it in the lab, it is best to aliquot into smaller portions to maintain consistent results over time and avoid repeated freeze-thaw cycles.
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Does the antibody show any cross-reactivity with other bacteria?
A: Based on the product details and our own tests, this antibody shows high specificity for the Streptococcus pneumoniae 23F antigen. There is little to no cross-reactivity with other bacterial antigens, which is great because it reduces confusion in experiments. This makes it easier for any researcher to know that a positive signal is really coming from the 23F antigen, which is essential for precise research.
View the frequently asked questions answered by Creative Biolabs Support.
Cite This Product
To accurately reference this product in your publication, please use the following citation information:
(Creative Biolabs Cat# PABX-173, RRID: AB_3111703)
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
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COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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See other products for "Clone 023. 102"
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See other products for "23F strep"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PSBL-001 | Recombinant Human Anti-23F strep Antibody scFv Fragment (C05) | WB, FuncS | scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PFBL-001 | Recombinant Human Anti-23F strep Antibody Fab Fragment (C05) | WB, FuncS | Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PABX-173-S (P) | Recombinant Human Anti-Streptococcus pneumoniae 23F Antibody scFv Fragment (023.102) | Neut, ELISA, FuncS | scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PABX-173-F (E) | Recombinant Human Anti-Streptococcus pneumoniae 23F Antibody Fab Fragment (023.102) | Neut, ELISA, FuncS | Fab |
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